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cell proliferation dye efluor 450  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cell proliferation dye efluor 450
    Cell Proliferation Dye Efluor 450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+proliferation+dye+efluor%C2%AE+450/cell+proliferation+dye+efluor+670/pm40605638-371-5-10
    Average 90 stars, based on 1 article reviews
    cell proliferation dye efluor 450 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.

    Concentration Assay:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.

    Immunopeptidomics:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.

    Transduction:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.

    Isolation:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.

    Labeling:

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures.
    Article Snippet: 3 × 106 PBMCwere stainedwith 10mMofCell ProliferationDye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 106 cells/ml in a final volume of 200ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flatwell culture plates.

    Article Title: Specific types of male infertility are correlated with T cell exhaustion or senescence signatures
    Article Snippet: 3 × 10 6 PBMC were stained with 10 mM of Cell Proliferation Dye eFluor® 450 (Invitrogen, CA, USA), according to the manufacturer’s instructions, and plated at a concentration of 10 6 cells/ml in a final volume of 200 ml of completed X-VIVO 15 (Lonza, NC, USA) in 96-flat well culture plates.

    Article Title: MALT1 inhibition suppresses antigen-specific T cell responses.
    Article Snippet: Isolated CD4+ CD25- T-cells were then labeled with Cell Proliferation Dye eFluor® 450 per manufacturer’s instructions (eBioscience).

    Article Title: Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release
    Article Snippet: For recording proliferation, CAR T cells were labeled with 10 μM “Cell Proliferation Dye eFluor® 450” (ThermoFisher) before stimulation.

    Article Title: A GLB1 transgene with enhanced therapeutic potential for the preclinical development of ex-vivo gene therapy to treat mucopolysaccharidosis type IVB
    Article Snippet: To evaluate the enhanced b-GAL immunogenicity, PBMCs were stained with Cell Proliferation Dye eFluor® 450 (eBioscience, 65-0842-85) according to manufacturer’s instructions and plated in X-VIVO 15 (Lonza) supplemented with 5% human serum (Sigma Aldrich) and 100 U/mL penicillin/streptomycin (Lonza) (hereafter T cell medium) alone or in the presence of different volumes of T cell medium conditioned by HEK 293T cells transduced with LV-hGLB1 or LV-enhGLB1-1 or LV-enhGLB1-2.

    Article Title: Limited protection against early-life cytomegalovirus infection results from deficiency of cytotoxic CD8 T cells
    Article Snippet: Stainings with Cell Proliferation Dye eFluor® 450 or 670 (eBioscience) were performed according to the manufacturer instructions.



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